Multiplex PCR system for fungal pathogen detection

Multiplex Touchdown PCR for Rapid Typing of the Opportunistic Pathogen

Usefulness of Multiplex Real-Time PCR for Simultaneous Pathogen Detection and Resistance Profiling of Staphylococcal Bacteremia

Staphylococci are the leading cause of nosocomial blood stream infections. Fast and accurate identification of staphylococci and confirmation of their methicillin resistance are crucial for immediate treatment with effective antibiotics. A multiplex real-time PCR assay that targets mecA, femA specific for S. aureus, femA specific for S. epidermidis, 16S rRNA for universal bacteria, and 16S rRNA...

FilmArray, an Automated Nested Multiplex PCR System for Multi-Pathogen Detection: Development and Application to Respiratory Tract Infection

The ideal clinical diagnostic system should deliver rapid, sensitive, specific and reproducible results while minimizing the requirements for specialized laboratory facilities and skilled technicians. We describe an integrated diagnostic platform, the "FilmArray", which fully automates the detection and identification of multiple organisms from a single sample in about one hour. An unprocessed ...

A multiplex PCR assay for determination of mating type in isolates of the honey bee fungal pathogen, Ascosphaera apis

A multiplex PCR assay for determination of mating type in isolates of the honey bee fungal pathogen, Ascosphaera apis Kate Aronstein & Deanna Colby To cite this article: Kate Aronstein & Deanna Colby (2016): A multiplex PCR assay for determination of mating type in isolates of the honey bee fungal pathogen, Ascosphaera apis, Journal of Apicultural Research, DOI: 10.1080/00218839.2015.1109917 To...

PathogenMip Assay: A Multiplex Pathogen Detection Assay

The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection. We have demonstrated the utility of this assay with an initial set of 24 probes targeting the most clinically relevant HPV genotypes associated with cervical canc...